FIG 5.

Lanthanide analysis of Beijerinckiaceae bacterium RH AL1. Strain RH AL1 was grown with different concentrations (1 and 10 μM) of either lanthanum by itself or a cocktail of lanthanides (La, Ce, Nd, Dy, Ho, Er, Yb). The lanthanide content in harvested biomass was determined by ICP-QqQ-MS with three biological replicates. (A and B) The numbers of lanthanide ions per cell were calculated by linking the lanthanide content of biomass samples with the number of cells present in these samples. Cell numbers were determined by 16S rRNA gene-targeting quantitative PCR with three biological replicates and three technical replicates. Error bars reflect the standard deviation of the ICP-QqQ-MS measurements. (C) Ln³⁺-to-La ratios were calculated by dividing the respective values for ions per cell.