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Effects of the HBc CDM mutations on infection. HBV inocula were prepared from HepG2 cells cotransfected with the indicated WT or mutant HBc expression construct plus the HBc-defective replicon. HBV PF DNA was extracted from HepG2-NTCP cells 3 days after infection and measured by Southern blot analysis using a 32P-labeled HBV DNA probe.
