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Effects of the HBc CDM mutations on virion secretion. HepG2 cells were transfected as described in the legend to Fig. 5. Cell culture supernatant was collected on day 5 posttransfection. HBV particles in concentrated culture supernatant were resolved by agarose gel electrophoresis and transferred to a nitrocellulose membrane. HBV DNA in virions and naked capsids was detected by using the HBV DNA probe.
