TABLE 1.
Primers used for cloning
| PCR target | Primer sequence (5′→3′) |
|---|---|
| NSP1 NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGGAGAGCCTTGTCCCTGGTTTC |
| NSP1 FseI rev | CGCCGGGCCGGCCCCCTCCGTTAAGCTCACGCATGAG |
| NSP2 PasI for | TAGCCCCAGGGGCCACCATGGCATACACTCGCTATGTCGATAAC |
| NSP2 FseI rev | CGCCGGGCCGGCCACCGCCTTTGAGTGTGAAGGTATT |
| NSP3 PasI for | CTAGCCCCAGGGGCCACCATGGCACCAACAAAGGTTACTTTTGGT |
| NSP3 FseI rev | CGCCGGGCCGGCCACCACCCTTAAGTGCTATCTTTGT |
| NSP4 NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGAAAATTGTTAATAATTGGTTGAAG |
| NSP4 FseI rev | CGCCGGGCCGGCCCTGCAAAACAGCTGAGGTGATAGA |
| NSP5 NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGAGTGGTTTTAGAAAAATGGCATTC |
| NSP5 FseI rev | CGCCGGGCCGGCCTTGGAAAGTAACACCTGAGCATTG |
| NSP6 NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGAGTGCAGTGAAAAGAACAATCAAG |
| NSP6 FseI rev | CGCCGGGCCGGCCCTGTACAGTGGCTACTTTGATACA |
| NSP7 NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGTCTAAAATGTCAGATGTAAAGTGC |
| NSP7 FseI rev | CGCCGGGCCGGCCTTGTAAGGTTGCCCTGTTGTCCAG |
| NSP8 NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGGCTATAGCCTCAGAGTTTAGTTCC |
| NSP8 FseI rev | CGCCGGGCCGGCCCTGTAATTTGACAGCAGAATTGGC |
| NSP9 NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGAATAATGAGCTTAGTCCTGTTGCA |
| NSP9 FseI rev | CGCCGGGCCGGCCTTGTAGACGTACTGTGGCAGCTAA |
| NSP10 NheI for | AAGCTGGCTAGCCCCAGGGGCCACCATGGACTACAAAGACCATGACGGTGATTATAAAGATCATGACATCGACTACAAGGATGACGAGACAAGGGCCGGCCCGGCGGGAGCGGCGGGGCTGGTAATGCAACAGAAGTGCCT |
| NSP10 FseI rev | AAGCTTGGATCCTACTGAAGCATGGGTTCGCGGAGTTG |
| NSP12 PasI for | CTAGCCCCAGGGGCCACCATGTCAGCTGATGCACAATCGTTCCTAAACCGGGTTTGCGGTGTAAGTGCAGCC |
| NSP12 FseI rev | CGCCGGGCCGGCCCTGTAAGACTGTATGCGGTGTGTA |
| NSP13 NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGGCTGTTGGGGCTTGTGTTCTTTGC |
| NSP13 FseI rev | CGCCGGGCCGGCCTTGTAAAGTTGCCACATTCCTACG |
| NSP14 NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGGCTGAAAATGTAACAGGACTCTTT |
| NSP14 FseI rev | CGCCGGGCCGGCCCTGAAGTCTTGTAAAAGTGTTCCA |
| NSP15 NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGAGTTTAGAAAATGTGGCTTTTAAT |
| NSP15 FseI rev | CGCCGGGCCGGCCTTGTAATTTTGGGTAAAATGTTTC |
| NSP16 PasI for | CTAGCCCCAGGGGCCACCATGTCTAGTCAAGCGTGGCAACCGGGT |
| NSP16 FseI rev | CGCCGGGCCGGCCGTTGTTAACAAGAACATCACTAGA |
| Nucleocapsid NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGTCTGATAACGGACCCCAAAATCAGCGAAAT |
| Nucleocapsid FseI rev | CGCCGGGCCGGCCAGCCTGAGTTGAGTCAGCACTGCTC |
| ORF10 NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGGGCTATATAAACGTTTTCGCT |
| ORF10 FseI rev | CGCCGGGCCGGCCTGTGAGATTAAAGTTAACTACATC |
| ORF14 NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGCTGCAATCGTGCTACAACTTC |
| ORF14 FseI rev | CGCCGGGCCGGCCATCTGTCAAGCAGCAGCAAAGCAA |
| P6 NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGTTTCATCTCGTTGACTTTCAG |
| P6 FseI rev | CGCCGGGCCGGCCATCAATCTCCATTGGTTGCTCTTC |
| S NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGTTTGTTTTTCTTGTTTTATTG |
| S FseI rev | CGCCGGGCCGGCCTGTGTAATGTAATTTGACTCCTTT |
| E NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGTACTCATTCGTTTCGGAAGAG |
| E FseI rev | CGCCGGGCCGGCCGACCAGAAGATCAGGAACTCTAGA |
| M NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGGCAGATTCCAACGGTACTATT |
| M FseI rev | CGCCGGGCCGGCCCTGTACAAGCAAAGCAATATTGTC |
| NS3A NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGGATTTGTTTATGAGAATCTTC |
| NS3A FseI rev | CGCCGGGCCGGCCCAAAGGCACGCTAGTAGTCGTCGT |
| NS3B NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGATGCCAACTATTTTCTTTGCT |
| NS3B FseI rev | CGCCGGGCCGGCCCACTATTGTAAGGTATACAATAGT |
| NS7A NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGAAAATTATTCTTTTCTTGGCA |
| NS7A FseI rev | CGCCGGGCCGGCCTTCTGTCTTTCTTTTGAGTGTGAA |
| NS7B NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGATTGAACTTTCATTAATTGAC |
| NS7B FseI rev | CGCCGGGCCGGCCGGCGTGACAAGTTTCATTATGATC |
| NS8B NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGAAATTTCTTGTTTTCTTAGGA |
| NS8B FseI rev | CGCCGGGCCGGCCGATGAAATCTAAAACAACACGAAC |
| NS9B NheI for | AGCTGGCTAGCCCCAGGGGCCACCATGGACCCCAAAATCAGCGAAATG |
| NS9B FseI rev | CGCCGGGCCGGCCTTTTACCGTCACCACCACGAATTC |
| NSP1-KH164AA fusion for | TTTCAAGAAAACTGGAACACTGCAGCAAGCAGTGGTGTTACCCGTGAA |
| NSP1-KH164AA fusion rev | TTCACGGGTAACACCACTGCTTGCTGCAGTGTTCCAGTTTTCTTGAAA |