FIG 2.
CgFPR3 and CgFPR4 are required for virulence. (A) Biofilm formation assay. The wt, Cgfpr3Δ, Cgfpr4Δ, and Cgfpr3Δ4Δ strains were grown in RPMI 1640 medium containing 10% FBS in a 24-well polystyrene plate. After 48 h of incubation, the biofilm formed by yeast cells on polystyrene was stained with 0.4% crystal violet for 45 min, followed by three PBS washes. After destaining with 95% ethanol, the biofilm mass was measured by monitoring the absorbance at 595 nm. Data (means ± SEM; n = 3 to 4) represent biofilm ratios, which were calculated by dividing the absorbance units of mutants by those of the wt strain (considered 1.0). (B) Mouse infection assay. BALB/c mice were infected with the indicated C. glabrata strains intravenously and sacrificed 7 days after infection. Four organs, kidneys, liver, spleen, and brain, were harvested and homogenized in PBS. The homogenates were appropriately diluted and plated onto YPD medium containing penicillin and streptomycin. The CFU recovered from each organ of the individual mice are plotted. The individual mouse organ CFU are represented by diamonds, while bars mark the CFU geometric means (n = 8 to 9) for each organ. *, P < 0.05 (by a Mann-Whitney U test).