FIG 5.

TpdA (VP1881) modulates swimming motility in soft agar. Scatterplots representing the diameter of the migration halo in soft agar (0.3%) plates containing 0.1 mM IPTG, of at least 9 biological replicates from each strain of interest are shown. The horizontal line represents the mean diameter of migration. Overexpression of TpdA and its variants was achieved using expression constructs that contain an IPTG-inducible promoter. The empty plasmid pMMB67EH-Gm (pMMB) was used as a negative control. Means were compared to the WT strain harboring the pMMB plasmid, using an ordinary one-way ANOVA test followed by a Dunnett’s multiple-comparison test. Adjusted P values of ≤0.05 were deemed statistically significant. ****, P ≤ 0.0001.