FIG 6.
TpdA modulates the expression of the polar flagellar gene flaA. Shown are scatterplots of relative light units (RLU) from at least 5 biological replicates from each strain of interest harboring the transcriptional fusion pBBR-PflaA-lux, grown to the exponential phase (A) or the stationary phase (B) in the presence of 0.1 mM IPTG. RLU are arbitrary light units per ml divided by the optical density at 600 nm. The RLU value is directly proportional to the activity of the PflaA promoter. The horizontal line represents the mean RLU value. Overexpression of TpdA and its variants was achieved using expression constructs that contain an IPTG-inducible promoter. The empty plasmid pMMB67EH-Gm (pMMB) was used as a negative control. Means were compared to the WT using Brown-Forsythe and Welch ANOVA tests followed by Dunnett’s T3 multiple-comparison test. Adjusted P values of ≤0.05 were deemed statistically significant. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.