TABLE 3.
Summary of resistance-associated genes queried in P. aeruginosa isolates
| Gene | Description | Data for straina: |
||||||
|---|---|---|---|---|---|---|---|---|
| ABD11(32) | ABD61(4) | ABD62(4) | ABD63(4) | AED11(32) | ACD21(32) | ACD23(32) | ||
| aph(3′)-II/XV | Aminoglycoside 3′-phosphotransferase (EC 2.7.1.95) | WT | WT | WT | WT | WT | WT | WT |
| catB | Chloramphenicol O-acetyltransferase (EC 2.3.1.28) | WT | WT | WT | WT | WT | WT | WT |
| bla OXA-50 | Class D β-lactamase (EC 3.5.2.6), OXA-50 family | WT | WT | WT | WT | WT | WT | WT |
| fosA | Fosfomycin resistance protein FosA | WT | WT | WT | WT | WT | WT | WT |
| bla PDC | Class C β-lactamase (EC 3.5.2.6), PDC family | Δ208–214 | WT | WT | WT | WT | WT | WT |
| ampDh2 | N-Acetylmuramoyl-l-alanine amidase (EC 3.5.1.28) (1,6-anhydro-N-acetylmuramyl-L-alanine amidase) | WT | WT | WT | WT | WT | WT | WT |
| ampDh3 | N-Acetylmuramoyl-l-alanine amidase (EC 3.5.1.28) | WT | WT | WT | WT | WT | WT | WT |
| ampR | Transcriptional regulator AmpR, LysR family | WT | WT | WT | WT | WT | WT | WT |
| amgS | Alanine racemase (EC 5.1.1.1) | WT | WT | WT | WT | WT | WT | WT |
| clpa | ATP-dependent Clp protease, ATP-binding subunit ClpA | WT | WT | WT | Wt | Δ1–240 | WT | WT |
| clpP | ATP-dependent Clp protease, proteolytic subunit ClpP (EC 3.4.21.92) | WT | WT | WT | WT | WT | WT | WT |
| colR | Two-component transcriptional response regulator, OmpR family | WT | WT | WT | WT | WT | WT | WT |
| colS | Two-component sensor ColS | WT | WT | WT | WT | WT | WT | WT |
| cprS | Putative two-component sensor | WT | WT | WT | WT | WT | FS, ins C at nt 806 | WT |
| ctpA | Carboxyl-terminal processing protease CtpA | WT | WT | WT | WT | WT | WT | WT |
| dacB | PBP-4, d-alanyl-d-alanine carboxypeptidase (EC 3.4.16.4) | Δ464–476 | Δ464–476 | Δ464–476 | Δ464–476 | Δ464–476 | Δ464–476 | Δ464–476 |
| ftsI | PBP-3 | WT | WT | WT | WT | WT | WT | WT |
| dnaJ | Chaperone protein DnaJ | WT | WT | WT | WT | Δ1–205 | WT | WT |
| dnaK | Chaperone protein DnaK | WT | WT | WT | WT | WT | WT | WT |
| flgF | Flagellar basal body rod protein FlgF | WT | WT | WT | WT | WT | WT | WT |
| fusA | Translation elongation factor G | WT | WT | WT | WT | WT | WT | WT |
| glnD | [Protein-PII] uridylyltransferase (EC 2.7.7.59), [protein-PII]-UMP uridylyl-removing enzyme | WT | WT | WT | WT | WT | WT | WT |
| glpt | Glycerol-3-phosphate transporter | WT | WT | WT | WT | WT | WT | WT |
| galU | UTP-glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) | WT | WT | WT | WT | WT | WT | WT |
| grpE | Heat shock protein GrpE | WT | WT | WT | WT | WT | WT | WT |
| gyra | DNA gyrase subunit A (EC 5.99.1.3) | WT | WT | WT | WT | WT | WT | WT |
| gyrB | DNA gyrase subunit B (EC 5.99.1.3) | WT | WT | WT | WT | WT | WT | WT |
| hfq | RNA-binding protein Hfq | WT | WT | WT | WT | WT | WT | WT |
| hmga | Homogentisate 1,2-dioxygenase (EC 1.13.11.5) | WT | WT | WT | WT | WT | WT | WT |
| infB | Translation initiation factor 2 | WT | WT | WT | WT | WT | WT | WT |
| mvaT | Transcriptional regulator MvaT | WT | WT | WT | WT | WT | WT | WT |
| mexA | Multidrug efflux system, membrane fusion component MexA | WT | WT | WT | WT | WT | WT | WT |
| mexB | Multidrug efflux system, inner membrane proton/drug antiporter (RND type) MexB | WT | WT | WT | WT | WT | WT | WT |
| mexR | Multidrug resistance operon repressor MexR, MarR family | WT | WT | WT | WT | WT | WT | WT |
| mexS | Negative regulation of protein secretion | WT | WT | WT | WT | WT | WT | Δ1–23 |
| mexT | Transcriptional regulator MexT | Δ105–112; FS, del G at nt 449 | Δ105–112 | Δ105–112 | Δ105–112 | NA | Δ105–112; FS, del G at nt 449 | Δ105–112; FS, del G at nt 449 |
| mexZ | Transcriptional repressor of mexXY operon MexZ | WT | WT | WT | WT | WT | WT | WT |
| mpl | UDP-N-acetylmuramate:l-alanyl-γ-d-glutamyl-meso-diaminopimelate ligase (EC 6.3.2.-) | WT | WT | WT | WT | WT | WT | WT |
| nalC | Negative regulation of transporter activity | Δ1–150 | WT | WT | WT | Δ1–150 | Δ1–150 | WT |
| nalD | Negative regulation of transport | WT | WT | WT | WT | WT | WT | WT |
| nfxB | Transcriptional regulator | WT | WT | WT | WT | WT | WT | WT |
| oprD | Outer membrane low-permeability porin, OprD family | WT | WT | WT | WT | WT | WT | WT |
| oprM | Multidrug efflux system, outer membrane factor lipoprotein OprM | WT | WT | WT | WT | WT | WT | WT |
| fgtA | Glycosyltransferases involved in cell wall biogenesis | WT | WT | WT | WT | Δ1–78 | WT | WT |
| parC | DNA topoisomerase IV subunit A (EC 5.99.1.3) | FS, ins G at nt 2177 | WT | WT | WT | FS, ins G at nt 2177 | FS, ins G at nt 2177 | WT |
| parE | DNA topoisomerase IV subunit B (EC 5.99.1.3) | WT | WT | WT | WT | WT | WT | WT |
| parR | Two-component response regulator | WT | WT | WT | WT | WT | WT | WT |
| parS | Two-component response regulator | WT | WT | WT | WT | WT | WT | WT |
| pcm | Protein-l-isoaspartate O-methyltransferase (EC 2.1.1.77) | WT | WT | WT | WT | WT | WT | WT |
| pepA | Cytosol aminopeptidase PepA (EC 3.4.11.1) | WT | WT | WT | WT | WT | WT | WT |
| phoQ | Two-component sensor | WT | WT | WT | WT | WT | WT | WT |
| pitA | Low-affinity inorganic phosphate transporter | WT | WT | WT | WT | WT | WT | WT |
| pmrA | Two-component regulator system response regulator | WT | WT | WT | WT | WT | WT | WT |
| pmrB | Two-component regulator system signal sensor kinase | WT | WT | WT | WT | WT | WT | WT |
| ppkA | Type VI secretion system serine/threonine protein kinase | WT | WT | WT | WT | WT | WT | WT |
| rplb | Large subunit ribosomal protein L2p (L8e) | WT | WT | WT | WT | WT | WT | WT |
| rpoB | DNA-directed RNA polymerase beta subunit (EC 2.7.7.6) | del at nt 391–399 | WT | WT | WT | WT | WT | Early stop, del G at nt 384 |
| spoT | Guanosine-3′,5′-bis(diphosphate) 3′-pyrophosphohydrolase | WT | WT | WT | WT | WT | WT | WT |
| yerD | Ferredoxin-dependent glutamate synthase | WT | WT | WT | WT | WT | WT | Δ1–118 |
| PA3271 | Probable two-component sensor | WT | FS, del GG at nt 2167–2168 | WT | WT | WT | WT | WT |
| PA14_45870 | Heavy metal sensor histidine kinase | WT | Δ228–234 | WT | WT | WT | WT | WT |
| PA14_45880 | Two-component response regulator | WT | WT | WT | WT | WT | WT | WT |
| PA14_45890 | Multidrug efflux system MdtABC-TolC, inner-membrane proton/drug antiporter MdtB-like | Δ1–127 | WT | WT | WT | WT | WT | WT |
aWT, wild type; FS, frameshift; ins, nucleotide insertion; del, nucleotide deletion; nt, nucleotide; NA, not applicable.