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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: Cytotherapy. 2021 Apr 5;23(8):694–703. doi: 10.1016/j.jcyt.2021.03.001

Figure 3.

Figure 3.

Identification of CD8-restricted T-cell epitopes

(A) IFN-γ production by T-cells in response to PRAME peptide pool stimulation. (B)(C) T-cell responses to single PRAME peptides, present in the mini-pools, were evaluated by IFN-γ ELISpot assay. (D) HLA-I-restricted epitopes (36, 37, 43, and 44) were determined by measuring IFN-γ and TNF-α release by CD8+ T-cells. (E) CD4+ T-cells showed no specificity for HLA-I-restricted epitopes (36, 37, 43, 44). (F) The minimal 9-mer peptides FPEPEAAQP and VEVLVDLFL were determined by IFN-γ ELISpot assay. (G) FPEPEAAQP HLA restriction was confirmed using anti-HLA-B*35 antibody.