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. 2021 Jul 27;12(8):742. doi: 10.1038/s41419-021-04005-y

Fig. 1. ETV7 expression modulates 5-FU sensitivity in breast cancer-derived MCF7 and T47D cells.

Fig. 1

A, B Western blot analysis to measure ETV7 protein expression in MCF7 (A) and T47D (B) cells stably over-expressing ETV7 or their empty control. HSP70 was used as loading control. Blots were cropped for clarity and conciseness of the presentation. C, D Cell Titer Glo assay for survival analysis upon 5-FU treatment in MCF7 (C) and T47D (D) cells over-expressing ETV7 and their empty control. The percentage of viable cells was calculated normalizing the luminescence measures on the DMSO treated sample. E Relative percentage of PI positive cells calculated as the difference of 5-FU and DMSO treated cells measured by Annexin V-FITC/PI staining of MCF7 Empty and MCF7 ETV7 cells treated with 5-FU 200 μM for 72 h. F RT-qPCR analysis of ABCB1, ABCC1, and ABCG2 expression in MCF7 Empty and MCF7 ETV7 cells. G Western blot analysis of the anti-apoptotic BCL-2 and survivin protein expression in MCF7 Empty and MCF7 ETV7 cells. Tubulin was used as loading control. Bars represent the averages and standard deviations of at least three independent experiments. On the right of each blot is indicated the approximate observed molecular weight. *p-value < 0.05; **p-value < 0.01.