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. 2021 Jul 27;12(8):742. doi: 10.1038/s41419-021-04005-y

Fig. 4. The over-expression of ETV7 causes the repression of a signature of IFN-response genes.

Fig. 4

A A Venn diagram showing the number of differentially expressed genes (DEGs at a False Discovery Rate (FDR) ≤ 0.05) in the comparison between MCF7 and T47D cells over-expressing ETV7 and their respective controls. B, C Gene Set Enrichment Analysis (GSEA) of MCF7 and T47D cells over-expressing ETV7 vs MCF7 and T47D controls. Enrichment plots for Type 1 Interferon response (B) and Type 2 Interferon response (C) gene sets of the Hallmark Collection. The Normalized Enrichment Score (NES) represents the degree of the enrichment of the gene set; the negative sign indicates that the gene set is downregulated in cells over-expressing ETV7. D Heatmaps showing the standardized expression level of genes comprising the ETV7-regulated IFN-responsive gene signature in MCF7 (left) and T47D (right) cells. E RT-qPCR experiments for the validation of genes of the ETV7-regulated IFN-responsive signature with a Fold Change (FC) < −2 in MCF7 Empty and ETV7 cells. Bars represent the averages and standard deviations of at least three biological replicates. F RT-qPCR analysis of the expression of a group of IFN-responsive genes in MCF7 cells transfected with ETV7 targeting siRNA #1 and siRNA #2 or the scrambled control. **p-value < 0.01; ***p-value < 0.001. G RT-qPCR analysis of normalized ETV7 expression relative to untreated control in MCF7 cells treated with 5 ng/ml IFN-β (red) or IFN-γ (blue) at different time points. H RT-qPCR analysis of the normalized expression of the genes regulated by ETV7 (IFI35, HERC6, APOL6) in MCF7 cells treated with 5 ng/ml IFN-β (red) or IFN-γ (blue) at different time points. Bars represent the averages and SEM of at least three biological replicates.