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. 2021 Jul 27;7:196. doi: 10.1038/s41420-021-00574-1

Fig. 2. Sensitivity to ferroptosis was determined by TRIB2 in liver cancer cells.

Fig. 2

A Endogenous TRIB2 protein expression level in Bel-7404 and SK-Hep1 cells, as measured by IB. TRIB2 expression before and after TRIB2 was knocked out in Bel-7404 cells (B) and overexpressed in SK-Hep1 cells (C), as measured by qPCR and IB. TRIB2-sg resistant TRIB2 was also simultaneously overexpressed in TRIB2−/− Bel-7404 cells for the rescue experiments. Cell death, lipid ROS generation, and cell viability were measured in Bel-7404 cell with TRIB2 knocked out in the presence or absence of TRIB2 reconstitution, or in SK-Hep1 cells with TRIB2 overexpressed following treating with RSL3 (5 µM, 12 h) or erastin (10 µM, 12 h) with or without DFO (25 µM, 12 h), as measured by Sytox green following flow cytometry (D, G), C11-BODIPY staining following flow cytometry (E, H) and a ATP detection kit (F, I). Data were analyzed by one-way ANOVA and expressed as mean ± SD from three independent experiments. NS non-significance; **P < 0.01; ***P < 0.001; ****P < 0.0001. Images of all the immunoblots are representative of three independent experiments.