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. 2021 Jul 27;7:196. doi: 10.1038/s41420-021-00574-1

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© The Author(s) 2021, corrected publication 2021

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Fig. 3 — AA and AdA in the membrane fractions were measured in Bel-7404 cells with TRIB2 knocked out with or without reconstitution of TRIB2 (A, C), and in SK-Hep1 cells with or without TRIB2 overexpression (B, D) in basal condition or following treating with RSL3 (5 µM, 12 h) or erastin (10 µM, 12 h). Membrane fractions were extracted by a Fatty Acid Extraction Kit, while AA and AdA were measured by ELISA-based kits. E, F TRIB2 decreased labile iron in liver cancer cells. Labile iron was measured in Bel-7404 and SK-Hep1 cells under the same conditions as those in A–D. Data were analyzed by one-way ANOVA and expressed as mean ± SD from three independent experiments. NS non-significance; ***P < 0.001; ****P < 0.0001.