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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Plant J. 2021 Jun 21;107(1):215–236. doi: 10.1111/tpj.15286

Figure 4. Steady state FPN3 transcript levels in split-roots.

Figure 4.

(A, B) Schematic overview of iron growth conditions prior to RNA extraction and comparison of FPN3 expression in split roots. Seedlings were grown under iron-sufficient (+Fe; A) or deficient (−Fe; B) conditions, then each root was transferred to iron-deficient or iron-sufficient medium for 0, 6, or 12 hours. The iron growth conditions of each split root are denoted as: +Fe/+Fe for growth in iron sufficient conditions followed by iron sufficiency treatment; +Fe/−Fe for growth in iron sufficient conditions followed by iron deficiency treatment; −Fe/+Fe for growth in iron deficient conditions followed by iron sufficiency treatment; −Fe/−Fe for growth in iron deficient conditions followed by iron deficiency treatment. Steady state FPN3 transcript level detected by RT-qPCR with roots from +Fe/+Fe condition was set at 1 for comparison and expression ratios were plotted. Significant differences compared to 0 hour within each condition were determined by one-way ANOVA followed by Dunnett’s test and denoted with different letters (p<0.05; **: p<0.01; n=5; error bars = SD).