Figure 4.

Blocking FcγIIIb decreases biomaterial-induced NET release on small but not large fiber biomaterials. (A and B) Fluorescent micrographs of neutrophils on the electrospun biomaterials at 3 hours after seeding. NETs are shown in red and nuclei in purple. Scale bars are 50 μm. (C and D) Percent NET release at 3 hours as quantified by the ELISA for NET-disassociated MPO. The quantification of percent NET release (n = 4) indicates that blocking FcγIIIb on the small fiber biomaterials significantly reduces NET formation whereas no effect is observed on the large fiber biomaterials. The data represent the mean ± standard deviation of three independent experiments with unique donors. * p < 0.05 was determined using an ANOVA and Holm-Sidak’s multiple comparisons test. Raw data is available in Supplementary Spreadsheet 1.