Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 27;12:4551. doi: 10.1038/s41467-021-24808-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a Snapshots of the browser showing representatives NIPBL ChIP-seq data in control fibroblasts in two different genomic regions. b NIPBL peaks distribution. Percentages of NIPBL peaks that colocalized with the indicated genomic features (black, GC > 60; blue, enhancer; purple, CpG Island; green, promoters). c Pie charts showing the distribution of NIPBL peaks located at CpG islands in promoters (red), enhancers (blue), or promoters + enhancers (violet) and others (green). d Plot representing the NIPBL peaks distribution with respect to the chromosome distance (pink) compared with random peak distribution (green). e Heatmap (left panel) and plot of mean read density NIPBL signals (right panel) showing a general loss of NIPBL signal around NIPBL peaks in Patient 3-derived cells (red) compared with the control (black). f Heatmap showing NIPBL signal distribution with respect to the DNA GC content and its presence into a CpG island and their corresponding signal in the patient (GC content < = 60%, pink; GC content > 60%, light blue; in CpG island, light red; not in CpG Island, blue). g Snapshots of the NIPBL peaks distribution in control (C) and CdLS Patient 3-derived cells (P3) at four gene clusters. CpG island positions are indicated in green boxes. h DNA methylation in CdLS-derived fibroblasts. (upper panel) Volcano plot representing the DNA methylation changes in control and CdLS-derived fibroblasts (significantly methylated samples are marked in blue and non-significant in red). Validation by bisulfite pyrosequencing was performed at six DMPs for HOXB3 (Control, n = 3, gray; Patients, n = 3, blue) and at five for HOXC4, 5, and 6 (Control, n = 3, gray; Patients, n = 4, blue) from three independent experiments (lower panel). Means were calculated for all the DMPs within a promoter and represented as DMRs. Two-sided unpaired student’s t-test (****p < 0.0001).