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. 2021 Jul 27;11:15302. doi: 10.1038/s41598-021-94709-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical analysis of muscle fiber type (A) Representative images are shown for type 2A, type 2B, type 1 muscle fiber, visualized with DAB (brown). The bar indicates 200 μm. Magnification: ×100. (B) Distribution of fiber types in gastrocnemius muscle. (C) Mean cross-sectional fiber area of type 2A, type 2B and type 1. US unstretched, St stretched. Values are expressed as mean ± SEM. *P < 0.05, n = 8/group.