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. 2021 Jul 27;12:4548. doi: 10.1038/s41467-021-24799-x

Fig. 2. Treatment with Pkd1-null cell-derived EVs/exosomes decreased the expression of Pkd1 gene but induced the activation of PKD-associated signaling pathways to promote cystic cell proliferation.

Fig. 2

a qRT-PCR analysis of Pkd1 mRNA expression in mIMCD3 cells treated with or without PN24 cell-derived EVs/exosomes. Data were analyzed from five experiments. b Western blot analysis of polycystin 1 (PC1) expression from whole-cell lysates of mIMCD3 cells treated with or without PN24 cell-derived EVs/exosomes. Data were analyzed from three experiments. c Western blot analysis of the phosphorylation and total proteins of AKT, mTOR, S6, Rb, STAT3, and ERK in mIMCD3 cells treated with or without PN24 cell-derived EVs/exosomes. d Western blot analysis of PCNA expression in mIMCD3 cells treated with or without PN24 cell-derived EVs/exosomes. Data were analyzed from three experiments. e Immunostaining for PCNA in mIMCD3 cells treated with or without PN24 cell-derived EVs/exosomes (40 μg/ml) for 48 h. Cells were costained with DAPI to visualize the nuclei. Scale bars, 100 μm. Data were analyzed from three experiments. All statistical data are represented as mean ± SEM in a, b, d, and e. P values by one-way ANOVA followed by Tukey’s post hoc test in a, b, d and by two-tailed unpaired t-tests in e are indicated.