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. 2021 Jul 27;12:4546. doi: 10.1038/s41467-021-24784-4

Fig. 8. WNK1 knockout in macrophages increases NLRP3 inflammasome activation in vivo.

Fig. 8

IL-1β release (a), number of infiltrating neutrophils (b), and percentage of neutrophils (c) were measured from peritoneal exudate of Wnk1+/+ LysMCre+ (WNK1 WT) and Wnk1flox/flox LysMCre+ (WNK1 KO) mice injected with 1 mg MSU for 6 h; n = 8 mice per group. P value for a is 0.002018; b is 0.00009; c is 0.00012. IL-1β release (d), number of infiltrating neutrophils (e), and percentage of neutrophils (f) were measured from peritoneal exudate of Wnk1+/+ LysMCre+ (WNK1 WT) and Wnk1flox/flox LysMCre+ (WNK1 KO) mice injected with 1 mg MSU for 16 h; n = 8 mice per group. P value for d is 0.01299; e is 0.04810; f is 0.04467. 3 mice per experiment were injected with PBS as a negative control. Error bars in af are presented as mean values ± standard deviation (S.D.). Two-sided Student’s t test, *p < 0.05, **p < 0.005, ***p < 0.0005.