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. 2021 Jul 27;12:4546. doi: 10.1038/s41467-021-24784-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — IL-1β (a) and LDH (b) release of culture supernatant of LPS-primed (4 h) primary Wnk1^flox/flox LysMCre⁺ (WNK1 KO) and Wnk1^flox/flox LysMCre⁺ Wnk4^−/− BMDMs (WNK1/4/ DKO) treated with ATP, dAdT transfection, imiquimod, MSU, and nigericin as indicated. P values in a are 0.3684, 0.1465, 0.5583, 0.0642, 0.1596, and 0.2239; b are 0.8798, 0.3554, 0.0879, 0.1037, 0.8199, and 0.2771. c Immunoblots of caspase-1 p20 released in culture supernatants (Sup) and procaspase-1 in cell lysates (Lys) of LPS-primed (4 h) primary Wnk1^flox/flox LysMCre⁺ (WNK1 KO) and Wnk1^flox/flox LysMCre⁺ Wnk4^−/− BMDMs (WNK1/4/ DKO) treated with ATP, dAdT transfection, imiquimod, MSU, and nigericin as indicated. Percentage of neutrophils (d), number of infiltrating neutrophils (e), and IL-1β release (f) were measured from peritoneal exudate of Wnk1^flox/flox LysMCre⁺ (WNK1 KO) and Wnk1^flox/flox LysMCre⁺ Wnk4^−/− BMDMs (WNK1/4/ DKO) mice injected with 1 mg MSU for 6 h; n = 8 mice per group. n = 8 mice per group. P value d is 0.5569, e is 0.5483, and f is 0.4089. 3 mice per experiment were injected with PBS as a negative control. Error bars in a, b, d–f are presented as mean values ± standard deviation (S.D.), with n = 3. Two-sided Student’s t test, n.s. not significant.