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. 2021 Jul 13;11(3):217–233. doi: 10.1007/s13534-021-00199-4

Fig. 8.

Fig. 8

a Nanoassemblies between Cas9 and AuNPs: The length of glutamate (E-tag) significantly plays a role in the self-assembly formation between Cas9En (glutamate-tagged Cas9) and ArgNPs (arginine-AuNPs); larger nanoassemblies are observed when the length of E-tag is increased. b Gene editing capability: Approximately 30% of indel efficiency (lane 1) has been observed in the targeted AAVS1 and PTEN gene, indicating an efficient gene editing as opposed to delivering Cas9E15-RNP alone (Lane 2) and cell only control (Lane 3). Arrowheads (red) designate the DNA cleavages. Reprinted with permission from American Chemical Society [98]. (Color figure online)