Fig. 6. Germline Dnmt3aR878H/+ mice have differentially expressed genes in hematopoietic cells.
a tSNE projections of scRNA-seq data from whole bone marrow samples from Dnmt3a+/+ (left, n = 2) and Dnmt3aR878H/+ (right, n = 2) mice at 1 month and 9 months of age, showing known populations defined by graph-based clustering and defined by ToppGene. b Population fractions associated with scRNA-seq data shown in Panel a by genotype and age. c and d Examples of differentially expressed genes identified in both humans and mice, and in more than one graph-based cluster, and associated with a differentially methylated region; tSNE projection, read counts per cell in all cells, and percentage of myeloid cells expressing Hoxb4 (panel c) and T-cells expressing Rasip1 (Panel d) are shown (Data are presented as mean values + /- SEM. P-values by two-tailed t test or Fisher’s exact test for ratios are indicated, n = 2 biologically independent samples per genotype). PMN polymorphonuclear leukocyte, GMP granulocyte-monocyte precursor.