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. 2021 Jul 14;9:705341. doi: 10.3389/fchem.2021.705341

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Copyright © 2021 Shu, Zhang, Chen, Guo, Li, Chen, Cao, Yan, Lu, Liu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Purification of Hp and its O-glycosylation status by lectin blot analysis. (A) Purified Hp from the sera of LC and HCC patients. M, marker; Hp-S, Hp standard; Hp-LC, purification of Hp from LC patients; Hp-HCC, purification of Hp from HCC patients. (B) Carbohydrate-binding specificities of five lectins (ACA, GSL-1, JAC, VVA, and WFA). (C) Lectin blot was applied to reveal the O-glycan levels of Hp. Hp from an equal volume of LC and HCC sera was used, and three biological repeats were performed. p-value of less than 0.05 showing statistical significance using nonparametric Mann–Whitney U tests. L, LC; H, HCC.