Fig. 2.
Autism spectrum disorder-like behavioral abnormalities in male juvenile offspring. (A) Grooming test. Male juvenile offspring after maternal glyphosate exposure showed the increased grooming time compared to control group. Data are shown as mean ± standard error of the mean (SEM) (n = 8). ***p < 0.01 compared to control group (Student t test). (B) Three chamber social interaction test. Left: Two-way ANOVA (glyphosate: F1,28 = 6.929, p = 0.014; stranger: F1,28 = 46.21, p < 0.001; interaction: F1,28 = 11.89, p = 0.002). Right: Two-way ANOVA (glyphosate: F1,28 = 5.098, p = 0.032; stranger: F1,28 = 12.36, p = 0.002; interaction: F1,28 = 6.609, p = 0.016). Data are shown as mean ± SEM (n = 8). **p < 0.01, ***p < 0.001 compared to control group. N.S., not significant.