TABLE 3.
Primer specificity (Cq values).
| Strains | rpoC-126-F/R |
QSF03-BTH-F/R |
rpoB-Fw1/Rev1 |
|||
| Cq valuesa | Range of Tm | Cq valuesa | Range of Tm | Cq valuesa | Range of Tm | |
| Brochothrix thermosphacta DSM 20171T | 15.32 ± 0.10 | 82–82.5 | 15.45 ± 0.06 | 81 | 14.67 ± 0.07 | 82 |
| Brochothrix thermosphacta 19/R/663 | 14.42 ± 0.33 | 82–82.5 | 14.63 ± 0.05 | 81 | 13.94 ± 0.07 | 82 |
| Brochothrix thermosphacta CD 340 | 16.43 ± 0.09 | 82–82.5 | 16.76 ± 0.05 | 81–81.5 | 16.43 ± 0.16 | 82–82.5 |
| Brochothrix thermosphacta EBP 3084 | 15.26 ± 0.22 | 82–82.5 | 15.44 ± 0.04 | 81 | 14.60 ± 0.18 | 82 |
| Brochothrix thermosphacta TAP 125 | 15.28 ± 0.18 | 82–82.5 | 15.44 ± 0.08 | 81 | 14.62 ± 0.14 | 82 |
| Brochothrix thermosphacta EBP 3017 | 14.82 ± 0.21 | 82–82.5 | 14.93 ± 0.06 | 81 | 14.00 ± 0.22 | 82 |
| Brochothrix campestris 4712T | 30.49 ± 0.23 | 81.5 | 31.03 ± 0.17 | 80.5–81 | 31.52 ± 0.30 | 82 |
| Carnobacterium maltaromaticum 19/R/671 | 34.60 ± 0.48 | 79.5–81.5 | 36.04 ± 1.10 | 80.5–82 | 35.54* | NA |
| Carnobacterium divergens NCDO 2763T | 35.79 ± 0.47 | 78.5–82.5 | 35.43 ± 0.65 | 80–83.5 | NA | NA |
| Enterococcus faecalis ATCC 19433T | 35.15 ± 0.68 | 79–81 | 35.02 ± 1.14 | 80.5–81 | 36.14 ± 1.39 | 81.5 |
| Enterococcus faecium CIP 54.33 | 34.57 ± 0.79 | 80–81 | 34.37 ± 0.67 | 79.5–80.5 | 34.74 ± 1.35 | 80.5–81 |
| Escherichia coli CIP 53.125 | 31.39 ± 0.60 | 87.5 | 36.88 ± 0.62 | 75.5–81 | NA | NA |
| Companilactobacillus alimentarius DSM 20181 | 34.77 ± 1.09 | 73.5–81.5 | 36.14 ± 0.74 | 80.5–81 | 36.02 ± 0.76 | 82 |
| Latilactobacillus sakei subsp. sakei DSM 20017T | 35.59 ± 0.63 | 79.5–81.5 | 36.12 ± 1.19 | 81–83.5 | NA | NA |
| Lactococcus piscium 19/R/684 | 34.56 ± 0.92 | 79.5–82 | 35.46 ± 1.44 | 80.5–81.5 | 35.05 ± 0.84 | 82 |
| Lactococcus raffinolactis Oniris MIP 2453 | 36.22 ± 0.10 | 79.5–80 | NA | NA | NA | NA |
| Listeria monocytogenes DSM 12464T | 35.61 ± 0.66 | 79.5–83 | 34.12 ± 1.04 | 84–84.5 | 36.51 ± 0.99 | 82 |
| Listeria monocytogenes CIP 78.35 | 35.37 ± 0.49 | 79.5–81.5 | 33.39 ± 1.20 | 84 | 35.53* | NA |
| Listeria grayi CIP 68.18T | 365.09 ± 0.22 | 81.5 | 35.01 ± 1.26 | 80.5–81 | 36.50 ± 0.46 | 82 |
| Listeria innocua CIP 80.11T | 35.71 ± 0.62 | 79.5–83 | 36.32* | 81 | 37.07* | 76 |
| Photobacterium phosphoreum CIP 102511T | 34.59 ± 1.05 | 81.5–83 | 35.31 ± 1.48 | 80.5–81 | 36.47 ± 0.79 | 82 |
| Pseudomonas fluorescens CIP 69.13T | 34.35 ± 0.50 | 91 | NA | NA | NA | NA |
| Psychrobacter sp. 19/R/675 | 35.09 ± 0.40 | 80.5–82.5 | NA | NA | NA | NA |
| Serratia liquefaciens ATCC 27592T | 34.91 ± 0.30 | 82.5 | NA | NA | NA | NA |
| Shewanella baltica 19/R/670 | 32.55 ± 0.38 | 82–87 | NA | NA | NA | NA |
| Staphylococcus epidermidis CIP 68.21 | 29.48 ± 0.18 | 81.5–82 | 29.74 ± 0.22 | 80.5–81 | 29.98 ± 0.25 | 82 |
| NTC | 34.87 ± 1.99 | 72–83 | 38.58* | 76.50 | NA | NA |
aMean and standard deviation of three Cq values (obtained with 3 ng of DNA template). NTC, no template control; NA, no amplification. *no standard deviation calculated (only one Cq available).