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. 2021 Jul 14;12:654178. doi: 10.3389/fmicb.2021.654178

TABLE 4.

qPCR efficiency.

rpoC-126-F/R
QSF03-BTH-F/R
rpoB-Fw1/Rev1
Equationa y = Efficiency (%) r2 Equationa y = Efficiency (%) r2 Equationa y = Efficiency (%) r2
BHI culture extracts −3.5136x + 40.558 92.58 0.997 −3.5898x + 41.019 89.92 0.996 −3.5419x + 42.158 91.57 0.990
Diluted DNA extracts −3.3845 + 40.947 97.45 1 −3.5074x + 41.542 92.80 0.998 −3.6035x + 41.882 89.46 0.995
Smoked salmon tissue homogenate −3.2408x + 44.396 103.50 0.998 Not determined −3.5789 + 46.805 90.29 0.998

aequations derived from 3 replicates.