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. 2021 Jul 28;562:149–157. doi: 10.1016/j.virol.2021.07.011

Table 1.

List of the six overlapping ORFs detected in SARS-CoV-2.

ORF name Type of experimental evidence for expression or function Type of computational method for detection
ORF2b Ribosome profiling (Finkel et al., 2021), immunopeptidomics (Weingarten-Gabbay et al., 2020)
ORF3b Interferon antagonist, when expressed from a plasmid in Sendai-virus infected cells (Konno et al., 2020)
ORF3c Ribosome profiling (Finkel et al., 2021) Synplot2 (Firth, 2014, 2020), PhyloCSF (Lin et al., 2011; Jungreis et al., 2021b)
ORF3d Ribosome profiling (Finkel et al., 2021), antibodies (Hachim et al., 2020) Codon permutation method (Schlub et al., 2018; Nelson et al., 2020b), sequence-composition method (Pavesi, 2020)
ORF9b Ribosome profiling (Finkel et al., 2021), immunopeptidomics (Weingarten-Gabbay et al., 2020), suppressor of interferon response (Jiang et al., 2020) GOFIX (Michel et al., 2020), PhyloCSF (Lin et al., 2011; Jungreis et al., 2021b)
ORF9c Suppressor of antiviral response, when expressed from a plasmid in transfected cells (Dominguez Andres et al., 2020) GOFIX (Michel et al., 2020), sequence-composition method (Pavesi, 2020)