Fig. 4.
Human Primary B cells isolated from human tonsil. a Expression of Rel-A, Rel-B, cRel, NFkB1/p105, p50, NFkB2/p100 and p52 proteins in naïve B cells (NBC), centroblasts (CB) and centrocytes (CC) was determined via western blotting. b Single-locus ChIP showed both Rel-B and cRel enrichment on CDK6 and CCND3 promoters. Error bars mean SD. Three independent experiments were performed. This is a representative assay. c Qt-PCR showed enhanced CDK6 and CCND3 mRNA expression in centroblasts compared to that in naïve B cells and centrocytes. Regarding BCL2 mRNA the lowest level was found in centroblasts. Error bars mean SD. Three independent experiments were performed in triplicates. This is a representative assay