图 2.

ANXA2促进CCL18诱导的肺腺癌细胞趋化运动能力。A：在以Scrabble siRNA作为对照（Scr/A549）和两组稳定的siRNA（SiANXA2#1/A549和SiANXA2#2/A549）转染A549细胞后检测ANXA2蛋白的表达水平；B：用CCL18刺激Scr/A549和SiANXA2#2/A549细胞，进行趋化反应的比较。β-actin作为阴性对照。每独立实验至少重复3次, P < 0.05，差异有统计学意义；C：在加入或不加入10 ng/mL CCL18刺激的情况下，分析Scr/A549和SiANXA2#2/A549细胞的侵袭能力，P < 0.05。以×400放大倍数捕获图像，比例尺：20 μm。

ANXA2 promotes the chemotaxis of lung adenocarcinoma cells induced by CCL18. A: Two stable siRNAs (SiANXA2#1 and SiANXA2#2) were transfected into A549 cells respectively, scramble siRNA as a control (Scr). The ANXA2 protein expression level was detected by Western blot analysis. β-actin was used as a negative control; B: Comparison of chemotactic response with rCCL18 stimulation in Scr/A549 and SiANXA2#2/A549 cells. Each independent experiment was repeated at least three times, P < 0.05, the difference was statistically significant; C: Analyze the invasion ability of Scr/A549 and SiANXA2#2/A549 cells with or without rCCL18 stimulation of 10 ng/mL, P < 0.05. Capture images at ×400 magnification. Scale bar: 20 μm.