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. Author manuscript; available in PMC: 2021 Nov 9.
Published in final edited form as: ACS Infect Dis. 2021 Mar 25;7(7):1969–1984. doi: 10.1021/acsinfecdis.1c00055

Table 1.

Minimum inhibitory concentrations (MICs) and NgCA Ki values for AZM-based analogs against N. gonorrhoeae 181

graphic file with name nihms-1722040-t0009.jpg

AZI = azithromycin, AZM = acetazolamide.

a

MIC values against N. gonorrhoeae strain CDC 181 in μg/mL.

b

Fluorescence-polarization competition Ki values determined from the mean of one experiment performed in triplicate and IC50 values input into equation from Nikolovska-Coleska et al49. Based on the calculation theory and assay parameters the values obtained have a reliable lower limit of 33 nM for IC50. Any IC50 value < 33 nM unable to generate accurate Ki and thus is listed as not determined, n.d.

c

IC50 values obtained from FP competition assay presented in Table S2 and expressed as both mean and as 95% confidence interval to demonstrate precision.

d

Catalytic CO2 hydration assay Ki determined from the mean of one experiment in triplicate and IC50 values entered into Cheng-Prusoff equation82. Values reported are ± standard error of the mean.