. Author manuscript; available in PMC: 2021 Nov 9.

Published in final edited form as: ACS Infect Dis. 2021 Mar 25;7(7):1969–1984. doi: 10.1021/acsinfecdis.1c00055

Table 4.

Inhibitory constants for analogs against NgCA and hCAs

	CO₂ Hydration K_I (nM)^a				CO₂ Hydration K_I (nM)^a				CO₂ Hydration K_I (nM)^a
Cpd	NgCA	hCA I	hCA II	Cpd	NgCA	hCA I	hCA II	Cpd	NgCA	hCA I	hCA II
AZM	74.1 ± 3.2	250 ± 11	12.5 ± 0.8	12	14.1 ± 0.8	76.5 ± 3.6	26.2 ± 1.4	24	45.2 ± 3.0	64.7 ± 4.5	58.1 ± 2.2
1	73.2 ± 3.9	8,600^b ± 300	60^b ± 3	13	9.8 ± 0.4	63.3 ± 3.7	20.2 ± 1.5	25	61.4 ± 4.9	53.4 ± 2.8	20.9 ± 1.6
2	59.8 ± 2.1	235 ± 14	37.2 ± 1.9	14	75.8 ± 5.0	117 ± 9.4	47.7 ± 3.2	26	29.9 ± 1.4	14^c ± 0.9	0.9^c ± 0.04
3	41.0 ± 3.0	180 ± 6	30.9 ± 1.3	15	8.7 ± 0.7	152 ± 13	26 ± 1.8	27	63.6 ± 2.4	9.6^c ± 0.8	1.6^c ± 0.1
4	30.5 ± 1.1	167 ± 6.9	9.5 ± 0.8	16	78.6 ± 5.2	109 ± 7	29.0 ± 1.5	28	73.9 ± 3.0	701 ± 34	47.2 ± 4.1
5	27.5 ± 1.8	213 ± 14	22.3 ± 1.9	17	29.3 ± 2.1	372 ± 19	7.6 ± 0.6	29	42.1 ± 2.9	1,135 ± 98	78.4 ± 4.7
6	87.9 ± 5.0	215 ± 17	55.6 ± 3.7	18	5.8 ± 0.3	77.8 ± 6.9	4.9 ±0.3	30	62.0 ± 3.9	1,623 ± 126	104 ± 9.1
7	17.7 ± 0.9	367 ± 14	23.6 ± 1.4	19	34.6 ± 2.5	86.3 ± 5.9	19.4 ± 1.1	31	>10,000	n.d.	n.d.
8	9.1 ± 0.6	231 ± 15	7.3 ± 0.60	20	36.6 ± 2.0	58.7 ± 4.1	24.5 ± 1.7	32	632 ± 41	465 ± 11	97.2 ± 11
9	8.5 ± 0.4	328 ± 14	22.7 ± 1.8	21	6.7 ± 0.4	190 ± 18	10.2 ± 0.7	33	439 ± 41	1,331 ± 11	67.7 ± 11
10	15.2 ± 0.6	157 ± 13	24.6 ± 1.3	22	0.70 ± 0.05	945 ± 37	0.32 ± 0.02
11	42.3 ± 1.6	125 ± 11	41.5 ± 2.0	23	8.3 ± 0.4	855 ± 76	8.1 ± 0.70

AZM = acetazolamide;

Catalytic CO₂ hydration assay K_i determined from the mean of one experiment performed in triplicate and IC₅₀ values entered into Cheng-Prusoff equation. Values reported are ± standard error of the mean.

Previously reported by Di Cesare Mannelli et al.⁸¹

Previously reported by Turkmen et al.⁸⁰