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. 2021 Jul 14;12:696534. doi: 10.3389/fmicb.2021.696534

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Copyright © 2021 Calapa, Mulford, Rieman, Senko, Auler, Parker and Barton.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fe(III) reduction and changes in sulfate and pH in the column experiments. (A,B) Columns were operated semi-continuously, and sulfate and pH were measured in each pore volume (four volumes) recovered after each static incubation. The column was disassembled after two column volumes at day 63 for post mortem analysis. Error bars represent one standard deviation of triplicate columns. The concentration of dissolved Fe(II) is shown at pH 4.75 (A) and 6.8 (B). Sulfate concentrations (black) and pH (blue) in column effluents are shown in panels (C) (pH 4.75) and (D) (pH 6.8). The values for uninoculated columns are shown with open circles, with the sub muros-inoculated columns represented by closed circles.