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Comparison of decoupling modes in 13C-detected HNCOCA. 15N, 13Cα planes acquired with (A) COMB decoupling of both 15N and 2H, (B) 2H decoupling only, and (C) 15N decoupling only. The data were acquired as 2D experiments, and the 13C-13C couplings between C’-Cα and Cα-Cβ were removed with a double IPAP scheme. The insets provide a clearer visualization of peak shapes. The dashed lines correspond to traces shown to the right of each panel.
