Figure 2.

TβRI-TβRII-Fc chimeric receptor inhibits the expression of TGF-β direct target genes in B16 melanoma cells. B16 cells were incubated for 4 h in the absence (−) or presence of TGF-β isoforms [(TGF-β1 (Tβ1), TGF-β2 (Tβ2), or TGF-β3 (Tβ3) (3 ng/ml)] in combination with a vehicle (Control), 10 µM SB431542 or conditioned media from 293T cells containing Fc chimeric receptors (Control-Fc, TβRII-Fc, or TβRI-TβRII-Fc). The expression levels of (A) TMEPAI and (B) PAI-1 were determined through reverse transcription-quantitative PCR analysis. Each experiment was performed in triplicate and repeated twice. All data are normalized to the expression of β-actin. Error bars, SD. ***P<0.001. TβRI, TGF-β type I receptor; TβRII, TGF-β type II receptor; TGF-β, transforming growth factor-β; NS, not significant; TMEPAI, transmembrane prostate androgen-induced protein; PAI-1, plasminogen activator inhibitor-1.