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. 2021 Jul 14;12:708480. doi: 10.3389/fmicb.2021.708480

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Copyright © 2021 Ding, Zhao, Ban, Li, Venkataram Prasad, Gu and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Product profile of purified SdG5A. (A) HPAEC-PAD chromatograms of maltooligosaccharides standards (blue) and hydrolysis products of corn starch by SdG5A (pink). Product proportions calculated from the chromatogram are listed in the table. Substrates (2%, w/w) were hydrolyzed by the purified enzyme (2.0 U g^{– 1} dry weight of starch) at 4°C for 4 h. (B) Product composition arising from the hydrolysis of corn starch by SdG5A at different temperatures. Substrates (10%) were hydrolyzed by the purified enzyme (2.0 U g^{– 1} dry weight of starch). Each value represents the mean of three independent measurements (mean ± standard derivation).