TABLE 1.

qPCR target genes, primer/probe sequences, and reaction conditions for MST candidates Swan_1, Swan_2, and Swan_3 targeting swans, and Oyscab targeting oystercatchers.

Marker	Primers/probe	Sequences (5’-3’)	Product size (bp)	Reaction conditions	Sensitivity (n of samples)/specificity (n of samples)
Swan_2	Swan_2F	GTAATACGTAGGGGGCAAG	135	1 cycle of 10 min at 95°C and 40 cycles of 15 s at 95°C and 1 min at 60°C	Sensitivity of 75% (n = 16 swan feces) and a specificity of 90.2% (n = 116 samples)
	Swan_2R	TCTCCTGTACTCAAGTTTAAC
	Swan_2P	(FAM)-TACGCATTTCACCGCTACAC-(TAMRA)
Swan_1	Swan_1F	GCGGTTTAACAAGTCAGGAG	73	na	Sensitivity 50% (n = 6 target samples); specificity 40% (n = 5 non-target samples)
	Swan_1R	TACTCAAGTTTAACAGTTTCAAAA
Swan_3	Swan_3F	GGCGGTTTAACAAGTCAGGA	118	na	Sensitivity 83% (n = 6 target samples); specificity 0% (n = 5 non-target samples)
	Swan_3R	TACGCATTTCACCGCTACAC
	Swan_3P	(FAM)-ATAGTAAGCTTTTGAAACTGTTAA-(TAMRA)
Oyscab	OYSCAB_F	AAACTCTACGTGTAGGGTCT	206	na	Sensitivity 71% (n = 24 target samples); specificity 91% (n = 22 non-target samples
	OYSCAB_R	TCAACCGCACTCAAGTACG

na, not applicable; F, forward; R, reverse; P, probe.