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. 2021 Jul 18;42(4):514–524. doi: 10.24272/j.issn.2095-8137.2021.041

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Editorial Office of Zoological Research, Kunming Institute of Zoology, Chinese Academy of Sciences

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Atf6 and Perk signaling pathways are enhanced by heat stress in testes

A, B: mRNA expression of ERS-related genes (Grp78, Ire1, Perk and Atf6) in testes at 6, 12, and 24 h after heat treatment. C: Immunofluorescence and TUNEL double staining showing spermatocyte apoptosis after 12 h of recovery from heat stress. D, E, F: Representative immunofluorescent images of pIRE1 (D), pPERK (E), and ATF6 (F) All images were merged from pIRE1, pPERK, or ATF6 (green) and Hoechst 33342-stained nuclei (blue). Scale bar: 200 μm. Con: Control. Data are mean±SD (n=3); statistical significance is expressed as: ^**: P<0.01. NS: Not significant.