Figure 4.

Knockdown of ATF6 and PERK relieves heat-induced damage

A: qRT-PCR of Perk and Atf6 mRNA expression in si-Perk and si-Ptf6 cell lines. B: Western blotting of PERK and ATF6 expression in si-Perk and si-Atf6 cell lines. C: Image J analysis of (B). D: qRT-PCR of apoptosis-related genes (Caspase12, Caspase3, and Chop) in si-Perk and si-ATF6 cell lines at 4 h after heat treatment for 2 h. E: Western blotting of apoptosis-related protein (CASPASE12, CASPASE3, CHOP) expression in si-Perk and si-ATF6 cell lines at 4 h after heat treatment for 2 h. F: Image J analysis of (E). Con: Control, hs: Heat stress. Data are mean±SD (n=3); statistical significance is expressed as: ^**: P<0.01.