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. Author manuscript; available in PMC: 2021 Oct 22.
Published in final edited form as: J Med Chem. 2020 Oct 15;63(20):11522–11547. doi: 10.1021/acs.jmedchem.0c00531

Figure 5.

Figure 5.

Determination of protein:ligand binding of compound 2 analogs to FGF14 and the CTD of Nav1.6 using protein thermal shift. The top 7 peptidomimetics were screening for binding to purified FGF14 or Nav1.6 C-terminal tail protein in 96-well plates (n = 4 replicates per compound) using the fluorescence-based PTS assay. Hits were defined as those compounds that resulted in a change in protein melting temperature (ΔTM) by ≥ ± 2 °C compared to protein alone and are highlighted as either red (hits against FGF14) or blue (hits against Nav1.6). Individual replicates and the mean ΔTM is shown for each condition.