Skip to main content
. Author manuscript; available in PMC: 2021 Oct 22.
Published in final edited form as: J Med Chem. 2020 Oct 15;63(20):11522–11547. doi: 10.1021/acs.jmedchem.0c00531

Table 7.

Characterization of FGF14:Nav1.6 binding in the presence of 21a by SPR.

KD (nM)* kon (M−1 s−1) koff (s−1)
FGF14:Nav1.6 228.50 ± 57.54 2.18×104 ± 1.6×102 1.71×10−3 ± 1.02×10−5
FGF14+21a:Nav1.6 1418.65 ± 134.7 5.88×104 ± 2.9×102 3.68×10−3 ± 6.70×10−5
*

The equilibrium dissociation constants (KD) were calculated using data shown in Figure 7. Each KD is an average of that calculated using the simplest Langmuir 1:1 interaction model (KD = koff/kon) and the steady-state saturation model. ND, non-determinable.