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. 2021 Jul 14;11:688461. doi: 10.3389/fonc.2021.688461

Figure 2.

Figure 2

ASP prompted apoptosis in OC cells. ASP increased the expression of Annexin V in the OVCAR5 and SKOV3 cells in a dose dependent manner after 18 hours of treatment (A). The cells were treated with the indicated concentration of ASP for 24 hours. Western blotting showed that ASP reduced the expression of BCL-XL and MCL-1 in both cells (B). The activities of cleaved caspase 3, 8 and 9 for the cells treated with different concentrations of ASP were significantly increased after 14 hours of treatment (C). OVCAR5 and SKOV3 cells were pretreated with VAD-FMK (10 µM) for 1 hour, followed by treatment with ASP for 18 and 48 hours. Pretreatment with Z-VAD-FMK partially blocked ASP’s effects on reducing MCL-1 expression and inducing cleaved caspase-3 activity in both cells (D, E). Blocking caspase pathways by Z-VAD-FMK in combination with ASP significantly rescued cell viability (F). All experiments were repeated three times. *p < 0.05, **p < 0.01.