Figure 3.

Induction of cell cycle G1 arrest by ASP is dose-dependent. The OVCAR5 and SKOV3 cells were incubated in the presence of the indicated doses of ASP for 48 hours. The cell cycle phase distribution was determined by Cellometer. ASP induced an arrest of cells at the G1 phase in a dose-dependent manner in both OVCAR5 and SKOV3 cells (A). To evaluate expression of cell cycle related proteins, the OVCAR5 and SKOV3 cells were treated with ASP for 24 hours. Western blotting results showed that the expression of CDK4 and CDK6 proteins were decreased after treatment with ASP (B). The results shown are one of three independent experiments.