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. 2021 Jul 14;11:688461. doi: 10.3389/fonc.2021.688461

Figure 6.

Figure 6

ASP inhibited adhesion and invasion. The OVCAR5 and SKOV3 were treated with ASP at a range of doses from 0.1−5 mg/ml. laminin-1 assay showed that treatment OVCAR5 and SKOV3 cells with ASP for 2 hours significantly reduced cell adhesion (A).Invasion was determined by transwell assay after 4 hours of treatment with ASP. ASP significantly reduced cell invasion in a dose-dependent manner in both cells (B). Migration was assessed by wound healing assay after treatment with ASP for 48 hours. The results showed that ASP reduced OC cell migration (C). Western blotting found that ASP reduced the expression of VEGF, Snail and Slug in both cell lines (D). The obese and lean KpB mice were treated with 200 mg/kg ASP for 4 weeks. ASP decreased serum VEGF products in both obese and lean mice (E). IHC results showed that ASP reduced VEGF expression in both obese and lean mice (F). *p < 0.05, **p < 0.01.