Figure 7.

ASP activated AMPK and inhibited the AKT/mTOR pathways. The OVCAR5 and SKOV3 ovarian cell lines were treated with ASP at indicated doses for 24 hours. The expression of phosphorylated AMPK, Akt and S6 was analyzed by western blotting. ASP increased the expression of phosphorylated AMPK and decreased the expression of phosphorylated S6 in both cell lines. ASP decreased the expression of phosphorylated AKT in the OVCAR5 cells and increased phosphorylated AKT expression in the SKOV3 cells (A). IHC results showed that ASP increased phosphorylated AMPK and decreased phosphorylated S6 expression in the ovarian cancer tissues of obese and lean mice (B). *p < 0.05; **p < 0.01.