FIGURE 3.

Significantly altered proteins in pairwise comparisons between male vs. female and nicotine vs. control groups. (A) Summary of differentially regulated proteins in each pairwise comparison. (B) Volcano plots of log₂ fold change and –log₁₀ p-values are shown for each pairwise comparison in VTA and NAc shell after sub-chronic or chronic nicotine administration in C3H/HeJ or C57BL/6J mice. Black lines within the graph show the significance cutoffs as determined in Perseus with s0 = 0.5 and FDR < 0.05. Proteins meeting significance criteria are colored. Red indicates an increase in protein abundance, and green indicates a decrease in protein abundance.