FIGURE 4.

Comparison of commonly altered proteins across sex and treatment groups. Headings indicate the pairwise tests being compared, while in parentheses the blue and yellow text indicate the groups from which those pairwise tests are being drawn for comparison. Under each heading, a Venn diagram for each brain region shows the numbers of proteins that were uniquely (blue or yellow shape) or commonly (middle green shape) identified as significantly altered proteins in these comparisons. The commonly altered proteins, outlined in a dotted line, are further delineated to be commonly up-regulated in both pairwise tests across blue and yellow groups (both arrows up), commonly down-regulated (both arrows down), or commonly identified but altered in different directions (set of arrows in opposite directions). (A) Significantly altered proteins in MC vs. FC for each brain region compared across the C3H/HeJ and C57BL/6J mice used for the sub-chronic and chronic nicotine administration groups, respectively. (B) Significantly altered proteins after nicotine vs. control treatment in each sex compared across nicotine administration groups. NAc is not shown because there were no commonly altered proteins across nicotine administration groups. (C) Significantly altered proteins after sub-chronic or chronic nicotine administration compared across sex.