FIGURE 1.

PGE₂ induced Ca²⁺-dependent small intestine epithelial anion secretion. (A) Time courses of PGE₂ (10 μM) -evoked I _sc or vehicle (DMSO) when applied to the serosal (s) or mucosal (m) side of mice duodenal mucosal tissues (n ≥ 6). (B) When added to the serosal or mucosal side, vehicle or PGE₂-stimulated I _sc peak (n = 6). (C) Time courses of PGE₂-evoked I _sc after extracellular Ca²⁺ omission (0 Ca²⁺) from each side in duodenal mucosal tissues. (D) PGE₂-stimulated I _sc peak after Ca²⁺ omission from each side (n ≥ 6). Ctrl represents as the control in which normal extracellular Ca²⁺ was on both sides. Results are presented as mean ± SE. *p < 0.05, ****p < 0.0001, significantly different from the corresponding control by one-way ANOVA followed by Dunnett’s post-test.