FIGURE 12.

TRPV4-constituted SOCE mechanism in IECs. (A)Time courses of CPA (10 μM)-induced Ca²⁺ signaling in the extracellular Ca²⁺ omission (0 Ca, left) or not (2 Ca, right) in ICE-6 cells. (B) Time courses of CPA-evoked Ca²⁺ signaling with HC067047 (30 μM) pretreated in extracellular Ca²⁺ omission or not. (C) CPA-induced [Ca²⁺]_cyt mobilization with HC067047 (30 μM) pretreated in HEC-6 cells superfused with Ca²⁺ - containing solution (2 Ca). Results are presented as mean SE (n = 25–35 cells). (D) TPEN (50 μM) -evoked Ca²⁺ signaling in the extracellular Ca²⁺ omission (0 Ca, left) or not (2 Ca, right) in ICE-6 cells. (E) TPEN-evoked Ca²⁺ signaling with HC067047 (30 μM) pretreated in extracellular Ca²⁺ omission or not. (F) TPEN-induced [Ca²⁺]_cyt mobilization with HC067047 (30 μM) pretreated in HEC-6 cells superfused with Ca²⁺ - containing solution (2 Ca). Results are presented as mean SE (n = 25–35 cells) *p < 0.05 vs. corresponding control by Student’s unpaired, two-tailed t-test.