FIGURE 2.

PGE₂ induced Ca²⁺-dependent ion secretion by serosal SOCE mechanism in mice duodenum. (A) Time course of PGE₂ (10 μM) -evoked I _sc after mucosal or serosal application of 2-aminoethoxydiphenyl borate (2-APB, 100 μM, n = 6). (B) PGE₂-evoked I _sc peak after 2-APB was added to the serosal or mucosal side. (C) Time course of PGE₂-evoked I _sc after mucosal or serosal application of SKF-96365 (SKF, 30 μM, n = 6). (D) PGE₂-evoked I _sc peak after SKF-96365 was added to the serosal or mucosal side. (E) Time course of PGE₂-evoked duodenal I _sc after serosal application of GSK-7975A (GSK, 100 μM, n = 6). (F) PGE₂-evoked duodenal I _sc peak after serosal application of GSK-7975A. Ctrl represents the control without drug treatment. (G) Representative of the time course of PGE₂-stimulated duodenal I _sc after serosal addition of GdCl₃ (Gd³⁺, 30 μM, n = 6). (F) Summary of the effect of GdCl₃ on PGE₂-stimulated duodenal I _sc peak after serosal addition. Ctrl represents the control without drug treatment. Results are presented as mean ± SE. NS, no significant differences, *p < 0.05, ***p < 0.001, ****p < 0.0001 vs. corresponding control by Student’s unpaired, two-tailed t-test or one-way ANOVA followed Dunnett’s post-test.