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. 2021 Jul 14;11:687499. doi: 10.3389/fcimb.2021.687499

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Copyright © 2021 Santos, da Silva, Carneiro, de Lima, Fernandes, Silveira, Sant’Ana and Nogueira-Melo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ratio of globlet cells/100 of epithelial cells (EC) stained using the histochemical techniques of Alcian Blue (AB) pH 1.0, AB pH 2.5, and periodic acid-Schiff (PAS). Data is represented in box plots (median with 25 to 75 percentile), whiskers (2.5 to 97.5 percentile), and mean (+) (n = 6). *p < 0.05; **p < 0.01. Representative photomicrograph of the group 1655 at 90- and 120-days post infection showing goblet cells (black arrows) in each technique (AB pH 1.0 and 2.5 and PAS staining, 40× magnification, scale bar = 15 µm, Olympus BX50). CG, control group. 2903: group infected with MHOM/BR/1975/M2903. 2314: group infected with MHOM/BR/2003/2314. 1655: group infected with MHOM/BR/2000/1655.